Technology

The most advanced extraction technologies are used in the preparation of our precious ingredients and their bioactive compounds, to ensure the highest quality, potency, and efficacy.

Ultrasonic Isolation

A process that uses 20 kHz of ultrasonic frequency to extract our bioactive ingredients and protect them from absorbing the unwanted sound interference of the equipment.

Microencapsulation

An advanced nutraceutical and pharmaceutical processing technology that creates a barrier around the ingredients to protect them from external factors like air, light, moisture, or heat. This allows them to remain stable, retain their potency, and enhance their bioavailability.

Solvents-free/chemical-free Pulverization

A pulverization process that breaks down our ingredients and extracts our botanicals without any use of solvents or chemicals that are potentially harmful to our body and our environment.

Patented Enzymatic Process

A specific process that uses enzymes to unlock and unleash the bioactive compounds from our the botanical ingredients and extract the maximum best of what nature has to offer.

Freeze Drying Extraction

A premium method using freezing, lowering pressure and removing ice by sublimation (rather than using heat, like that of dehydrating), to remove water from our ingredients allows our botanical ingredients to maintain their quality and characteristics, including their innate aroma and flavor profile without damage.

Chromatographic purification

A method used to precisely isolate and purify different medicinal biomolecules and blends based on their structure and or compositions of the molecules.

Clinical Studies

There is a tremendous amount of science and research that goes into every one of Eightbillion's products. Sourcing the finest ingredients available and extracting them to elevate people and planet health and vitality.

  • A Prospective, Randomized Double-Blind, Placebo-Controlled Study of Safety and Efficacy of a High-Concentration Full-Spectrum Extract of Ashwagandha Root in Reducing Stress and Anxiety in Adults.

    Chandrasekhar K, Kapoor J, Anishetty S.Indian Journal of Psychological Medicine. 2012;34(3):255-262.
    DOI :10.4103/0253-7176.106022

  • Examining the effect of Withania somnifera supplementation on muscle strength and recovery: a randomized controlled trial.

    Sachin Wankhede, Deepak Langade, Kedar Joshi, Shymal R. Sinha & Sauvik Bhattacharyya (2015) Journal of the International Society of Sports Nutrition, 12:1, DOI: 10.1186/s12970-015-0104-9.

  • Efficacy of Ashwagandha (Withania somnifera [L.] Dunal) in improving cardiorespiratory endurance in healthy athletic adults.

    Choudhary, B., Shetty, A., & Langade, D. G. (2015). Ayu, 36(1), 63–68.

  • Efficacy and Safety of Ashwagandha (Withania somnifera (L.) Dunal) Root Extract in Improving Memory and Cognitive Functions.

    Dnyanraj Choudhary, Sauvik Bhattacharyya & Sekhar Bose (2017) Journal of Dietary Supplements, 14:6, 599-612.

  • Body Weight Management in Adults Under Chronic Stress Through Treatment with Ashwagandha Root Extract: A Double-Blind, Randomized, Placebo-Controlled Trial.

    Choudhary, D., Bhattacharyya, S., & Joshi, K. (2017). Journal of evidence-based complementary & alternative medicine, 22(1), 96–106.

  • Efficacy and Safety of Ashwagandha (Withania somnifera) Root Extract in Insomnia and Anxiety: A Double-blind, Randomized, Placebo-controlled Study.

    Langade, D., Kanchi, S., Salve, J., Debnath, K., & Ambegaokar, D. (2019). Cureus, 11(9), e5797.

  • Adaptogenic and Anxiolytic Effects of Ashwagandha Root Extract in Healthy Adults: A Double-blind, Randomized, Placebo-controlled Clinical Study.

    Salve, J., Pate, S., Debnath, K., & Langade, D. (2019). Cureus, 11(12), e6466.

  • Efficacy and Tolerability of Ashwagandha Root Extract in the Elderly for Improvement of General Well-being and Sleep: A Prospective, Randomized, Double-blind, Placebo-controlled Study.

    Kelgane, S. B., Salve, J., Sampara, P., & Debnath, K. (2020). Cureus, 12(2), e7083

  • Clinical evaluation of the pharmacological impact of ashwagandha root extract on sleep in healthy volunteers and insomnia patients: A double-blind, randomized, parallel-group, placebo-controlled study.

    Langade, D., Thakare, V., Kanchi, S., & Kelgane, S. (2020). Journal of Ethnopharmacology: 264:113276.

  • Safety of Ashwagandha Root Extract: A Randomized, Placebo-Controlled, study in Healthy Volunteers.

    Verma, N., Gupta, S. K., Tiwari, S., & Mishra, A. K. (2020). Complementary therapies in medicine, 57, 102642. Advance online publication

  • A double-blind, randomized, placebo-controlled trial on the effect of Ashwagandha (Withania somnifera Dunal.) root extract in improving cardiorespiratory endurance and recovery in healthy athletic adults.

    Tiwari, S., Gupta, S. K., & Pathak, A. K. (2021). Journal of ethnopharmacology, 113929. Advance online publication..

  • Effects of l-Theanine on Cognitive Function in Middle-Aged and Older Subjects: A Randomized Placebo-Controlled Study. 

    Baba Y, Inagaki S, Nakagawa S, Kaneko T, Kobayashi M, Takihara T. 

    J Med Food. 2021 Apr;24(4):333-341.
    DOI: 10.1089/jmf.2020.4803.
    Epub 2021 Mar 22.
    PMID: 33751906; PMCID: PMC8080935.

  • Effects of L-theanine-caffeine combination on sustained attention and inhibitory control among children with ADHD: a proof-of-concept neuroimaging RCT. 

    Kahathuduwa CN, Wakefield S, West BD, Blume J, Dassanayake TL, Weerasinghe VS, Mastergeorge A. Sci Rep. 2020 Aug 4;10(1):13072. doi: 10.1038/s41598-020-70037-7. PMID: 32753637; PMCID: PMC7403383.

  • Effects of L-Theanine Administration on Stress-Related Symptoms and Cognitive Functions in Healthy Adults: A Randomized Controlled Trial. 

    Hidese S, Ogawa S, Ota M, Ishida I, Yasukawa Z, Ozeki M, Kunugi H. 

    Nutrients. 2019 Oct 3;11(10):2362.
    DOI: 10.3390/nu11102362.
    PMID: 31623400; PMCID: PMC6836118.

  • Effect of l-theanine tablets in reducing stress-related emotional signs in cats: an open-label field study. 

    Dramard V, Kern L, Hofmans J, Rème CA, Nicolas CS, Chala V, Navarro C.

    Ir Vet J. 2018 Oct 9;71:21. doi: 10.1186/s13620-018-0130-4. PMID: 30338056; PMCID: PMC6178259.

  • Effects of chronic l-theanine administration in patients with major depressive disorder: an open-label study. 

    Hidese S, Ota M, Wakabayashi C, Noda T, Ozawa H, Okubo T, Kunugi H. 

    Acta Neuropsychiatr. 2017 Apr;29(2):72-79. doi: 10.1017/neu.2016.33. Epub 2016 Jul 11. PMID: 27396868.

  • In Search of a Safe Natural Sleep Aid. 

    Rao TP, Ozeki M, Juneja LR. 

    J Am Coll Nutr. 2015;34(5):436-47. doi: 10.1080/07315724.2014.926153. Epub 2015 Mar 11. PMID: 25759004.

  • L-theanine relieves positive, activation, and anxiety symptoms in patients with schizophrenia and schizoaffective disorder: an 8-week, randomized, double-blind, placebo-controlled, 2-center study. 

    Ritsner MS, Miodownik C, Ratner Y, Shleifer T, Mar M, Pintov L, Lerner V.

    J Clin Psychiatry. 2011 Jan;72(1):34-42. doi: 10.4088/JCP.09m05324gre. Epub 2010 Nov 30. PMID: 21208586.

  • Human disposition of L-theanine in tea or aqueous solution,

    P.C. van der Pijl, L. Chen, T.P.J. Mulder,Volume 2, Issue 4,

    Journal of Functional Foods, 2010, Pages 239-244, ISSN 1756-4646,

  • The effects of L-theanine on alpha-band oscillatory brain activity during a visuo-spatial attention task. 

    Gomez-Ramirez M, Kelly SP, Montesi JL, Foxe JJ. 

    Brain Topogr. 2009 Jun;22(1):44-51.
    DOI: 10.1007/s10548-008-0068-z.
    Epub 2008 Oct 9. PMID: 18841456.

  • L-theanine and caffeine in combination affect human cognition as evidenced by oscillatory alpha-band activity and attention task performance. 

    Kelly SP, Gomez-Ramirez M, Montesi JL, Foxe JJ. 

    J Nutr. 2008 Aug;138(8):1572S-1577S.
    DOI: 10.1093/jn/138.8.1572S.
    PMID: 18641209.

  • Protective effect of the green tea component, L-theanine on environmental toxins-induced neuronal cell death. 

    Cho HS, Kim S, Lee SY, Park JA, Kim SJ, Chun HS. 

    Neurotoxicology. 2008 Jul;29(4):656-62.
    DOI: 10.1016/j.neuro.2008.03.004.
    Epub 2008 Mar 20.
    PMID: 18452993.

  • Time for tea: mood, blood pressure and cognitive performance effects of caffeine and theanine administered alone and together. 

    Rogers PJ, Smith JE, Heatherley SV, Pleydell-Pearce CW. 

    Psychopharmacology (Berl). 2008 Jan;195(4):569-77.
    DOI: 10.1007/s00213-007-0938-1.
    Epub 2007 Sep 23.
    PMID: 17891480.

  • Whole Coffee Cherry Extract Improves Working Memory and Response Inhibition: Acute and Longitudinal Results from a Remote, Randomized, Double-Blind, Placebo-Controlled Clinical Trial. 

    Robinson JL, Hunter JM, Kern M, Rodas M, Jowers J, Robertson J, Wanalista C. 

    Nutrients. 2024 Jul 20;16(14):2348.
    DOI: 10.3390/nu16142348.
    PMID: 39064791; PMCID: PMC11279654.

  • Neurophysiological Effects of Whole Coffee Cherry Extract in Older Adults with Subjective Cognitive Impairment: A Randomized, Double-Blind, Placebo-Controlled, Cross-Over Pilot Study 

    Robinson JL, Yanes JA, Reid MA, Murphy JE, Busler JN, Mumford PW, Young KC, Pietrzkowski ZJ, Nemzer BV, Hunter JM, Beck DT. 

    Antioxidants (Basel). 2021 Jan 20;10(2):144.
    DOI: 10.3390/antiox10020144.
    PMID: 33498314; PMCID: PMC7909261.

  • Modulatory effect of coffee fruit extract on plasma levels of brain-derived neurotrophic factor in healthy subjects.

    Reyes-Izquierdo T, Nemzer B, Shu C, Huynh L, Argumedo R, Keller R, Pietrzkowski Z.  Br J Nutr. 2013 Aug 28;110(3):420-5.
    DOI: 10.1017/S0007114512005338.
    Epub 2013 Jan 14.
    PMID: 23312069.

  • Stimulatory Effect of Whole Coffee Fruit Concentrate Powder on Plasma Levels of Total and Exosomal Brain-Derived Neurotrophic Factor in Healthy Subjects: An Acute Within-Subject Clinical Study.

    January 2013 Food and Nutrition Sciences 4(09):984-990 Tania Reyes-Izquierdo, Ruby Argumedo, Cynthia Shu, Boris Nemzer, Zb Pietrzkowski.

  • Acute Low and Moderate Doses of a Caffeine-Free Polyphenol-Rich Coffeeberry Extract Improve Feelings of Alertness and Fatigue Resulting from the Performance of Fatiguing Cognitive Tasks. 

    Reed, R.A., Mitchell, E.S., Saunders, C. et al. Journal of Cognitive Enhancement 3, 193–206 (2019).
    https://doi.org/10.1007/s41465-018-0118-8

  • Cognitive short- and long-term effects of coffee cherry extract in older adults with mild cognitive decline 

    Jennifer L. Robinson, John M. Hunter, Tania Reyes-Izquierdo, Ruby Argumedo, Jessica Brizuela-Bastien, Robert Keller & Zbigniew J. Pietrzkowski 

    Aging, Neuropsychology, and Cognition, DOI: 10.1080/13825585.2019.1702622

  • A double-blind, placebo-controlled study evaluating the effects of caffeine and L-theanine both alone and in combination on cerebral blood flow, cognition and mood. 

    Psychopharmacology (Berl).  Dodd FL, Kennedy DO, Riby LM, Haskell-Ramsay CF.  2015 Jul;232(14):2563-76. DOI: 10.1007/s00213-015-3895-0.
    Epub 2015 Mar 13.
    PMID: 25761837; PMCID: PMC4480845.

  • Caffeine and theanine exert opposite effects on attention under emotional arousal.

    Giles GE, Mahoney CR, Brunyé TT, Taylor HA, Kanarek RB.  Can J Physiol Pharmacol. 2017 Jan;95(1):93-100.
    DOI: 10.1139/cjpp-2016-0498.
    PMID: 28044450.

  • The combined effects of L-theanine and caffeine on cognitive performance and mood.  

    Owen GN, Parnell H, De Bruin EA, Rycroft JA.  Nutr Neurosci. 2008 Aug;11(4):193-8.

    DOI: 10.1179/147683008X301513.
    PMID: 18681988.

  • The effects of L-theanine, caffeine and their combination on cognition and mood. 

    Haskell CF, Kennedy DO, Milne AL, Wesnes KA, Scholey AB. Biol Psychol. 2008 Feb;77(2):113-22.
    DOI: 10.1016/j.biopsycho.2007.09.008.
    Epub 2007 Sep 26.
    PMID: 18006208.

  • Acute effects of tea constituents L-theanine, caffeine, and epigallocatechin gallate on cognitive function and mood: a systematic review and meta-analysis.

    Camfield DA, Stough C, Farrimond J, Scholey AB.  Nutr Rev. 2014 Aug;72(8):507-22.
    DOI: 10.1111/nure.12120.
    Epub 2014 Jun 19.
    PMID: 24946991.

  • The effects of L-theanine, caffeine and their combination on cognition and mood. 

    Haskell CF, Kennedy DO, Milne AL, Wesnes KA, Scholey AB.  Biol Psychol. 2008 Feb;77(2):113-22.
    DOI: 10.1016/j.biopsycho.2007.09.008.
    Epub 2007 Sep 26.
    PMID: 18006208.

  • The combination of L-theanine and caffeine improves cognitive performance and increases subjective alertness.

    Giesbrecht T, Rycroft JA, Rowson MJ, De Bruin EA. Nutr Neurosci. 2010 Dec;13(6):283-90.
    DOI: 10.1179/147683010X12611460764840.
    PMID: 21040626.

  • Effects of l-theanine on attention and reaction time response.

    Akiko Higashiyama, Hla Hla Htay, Makoto Ozeki, Lekh R. Juneja, Mahendra P. Kapoor,

    Journal of Functional Foods, Volume 3, Issue 3, 2011, Pages 171-178, ISSN 1756-4646.

  • Assessing the effects of caffeine and theanine on the maintenance of vigilance during a sustained attention task. 

    Foxe JJ, Morie KP, Laud PJ, Rowson MJ, de Bruin EA, Kelly SP.  Neuropharmacology. 2012 Jun;62(7):2320-7.
    DOI: 10.1016/j.neuropharm.2012.01.020.
    Epub 2012 Feb 2.
    PMID: 22326943.

  • The effects of L-theanine (Suntheanine®) on objective sleep quality in boys with attention deficit hyperactivity disorder (ADHD): a randomized, double-blind, placebo-controlled clinical trial. 

    Lyon MR, Kapoor MP, Juneja LR. 

    Altern Med Rev. 2011 Dec;16(4):348-54.
    PMID: 22214254.

  • Effect of L-theanine on sensorimotor gating in healthy human subjects. 

    Ota M, Wakabayashi C, Matsuo J, Kinoshita Y, Hori H, Hattori K, Sasayama D, Teraishi T, Obu S, Ozawa H, Kunugi H. 

    Psychiatry Clin Neurosci. 2014 May;68(5):337-43.
    DOI: 10.1111/pcn.12134.
    Epub 2013 Dec 25.
    PMID: 24372613.

  • Anti-stress effect of theanine on students during pharmacy practice: positive correlation among salivary α-amylase activity, trait anxiety and subjective stress. 

    Unno K, Tanida N, Ishii N, Yamamoto H, Iguchi K, Hoshino M, Takeda A, Ozawa H, Ohkubo T, Juneja LR, Yamada H. 

    Pharmacol Biochem Behav. 2013 Oct; 111:128-35.
    DOI: 10.1016/j.pbb.2013.09.004.
    Epub 2013 Sep 16. PMID: 24051231.

  • Effects of L-theanine or caffeine intake on changes in blood pressure under physical and psychological stresses. 

    Yoto A, Motoki M, Murao S, Yokogoshi H. 

    J Physiol Anthropol. 2012 Oct 29;31(1):28.
    DOI: 10.1186/1880-6805-31-28.
    PMID: 23107346; PMCID: PMC3518171.

  • L-theanine, a natural constituent in tea, and its effect on mental state. 

    Nobre AC, Rao A, Owen GN. 

    Asia Pac J Clin Nutr. 2008;17 Suppl 1:167-8. PMID: 18296328.

  • Effects of beta-glucans from Coriolus versicolor on macrophage phagocytosis are related to the Akt and CK2/Ikaros.

    S.C. Kang, H.J. Koo, S. Park, J.D. Lim, Y.J. Kim, T. Kim, S. Namkoong, K.H. Jang, S. Pyo, S.A. Jang, E.H. Sohn, International journal of biological macromolecules, 57 (2013) 9-16.

  • Protective effect of polysaccharides on simulated microgravity-induced functional inhibition of human NK cells.

    T. Huyan, Q. Li, H. Yang, M.L. Jin, M.J. Zhang, L.J. Ye, J. Li, Q.S. Huang, D.C. Yin, Carbohydr Polym, 101 (2014) 819-827.

  • Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas.

    Y. Zhang, J. Li, F. Yu, X. He, Z. Yu, Fish & shellfish immunology, 34 (2013) 1071-1077.

  • 1,3-beta-glucan affects the balance of Th1/Th2 cytokines by promoting secretion of anti-inflammatory cytokines in vitro.

    Y. Chen, L. Dong, D. Weng, F. Liu, L. Song, C. Li, W. Tang, J. Chen, Molecular medicine reports, 8 (2013) 708-712.

  • Antitumor and anti-angiogenic activity of Ganoderma lucidum polysaccharides peptide. 

    Acta pharmacologica Sinica, Q.Z. Cao, Z.B. Lin,  25 (2004) 833-838.

  • Discharge of solubilized and Dectin-1-reactive beta-glucan from macrophage cells phagocytizing insoluble beta-glucan particles: involvement of reactive oxygen species (ROS)-driven degradation, 

    S. Hino, A. Kito, R. Yokoshima, R. Sugino, K. Oshima, T. Morita, T. Okajima, D. Nadano, K. Uchida, T. Matsuda, Biochemical and biophysical research communications, 421 (2012) 329-334.

  • Effects of Purified (1-->3)-beta-Glucan on Venous Ulcer Healing.

    S.D. Medeiros, S.L. Cordeiro, J.E. Cavalcanti, K.M. Melchuna, A.M. Lima, I.A. Filho, A.C. Medeiros, K.B. Rocha, E.M. Oliveira, E.D. Faria, G.L. Sassaki, H.A. Rocha, V.S. Sales, Effects of Purified (1-->3)-beta-Glucan on Venous Ulcer Healing, International journal of molecular sciences, 13 (2012) 8142-8158.

  • The Acute and Chronic Effects of Lion's Mane Mushroom Supplementation on Cognitive Function, Stress and Mood in Young Adults: A Double-Blind, Parallel Groups, Pilot Study. 

    Docherty S, Doughty FL, Smith EF.

    Nutrients. 2023 Nov 20;15(22):4842. doi: 10.3390/nu15224842. PMID: 38004235; PMCID: PMC10675414.

  • Therapeutic Potential of Hericium erinaceus for Depressive Disorder. 

    Chong PS, Fung ML, Wong KH, Lim LW. 

    Int J Mol Sci. 2019 Dec 25;21(1):163.
    DOI: 10.3390/ijms21010163.
    PMID: 31881712; PMCID: PMC6982118.

  • Neurotrophic and Neuroprotective Effects of Hericium erinaceus. 

    Szućko-Kociuba I, Trzeciak-Ryczek A, Kupnicka P, Chlubek D. 

    Int J Mol Sci. 2023 Nov 3;24(21):15960.
    DOI: 10.3390/ijms242115960.
    PMID: 37958943;
    PMCID: PMC10650066.